The first foundation of the Sisters of the Incarnate Word and Blessed Sacrament in México came at the invitation of the Bishop of Tabasco, Perfecto Amezquita y Gutierrez in 1894. Four Sisters from the Brownsville community made a long and hazardous trip to México, settling in San Juan Bautista. About seven years later they transferred their headquarters to Chilapa, then many years later to San Luis Potosi, and finally to México City.

In 1902, Mother Teresa Solis, Superior of the Tabasco community, and Sisters María Angela Porras and María Ignacia López, opened a foundation in Querétaro in response to Bishop Rafael Sabás Camacho's request. At first, they cared for orphans free of charge. Later, though, in order to support themselves, they opened a school to which parents contributed. During the Mexican Revolution of 1910, the Sisters were forced to separate. Some of the displaced members joined the communities of México City or Guadalajara. Similarly, the Sisters from the foundation in Matamoros, Tamaulipas, also disbanded and joined the Generalate of México City.

In 1929, under the leadership of Mother Concepción Solis, the three communities of Chilapa, Matehuala and México City joined together and formed a Generalate. Despite trials, persecution and poverty, the community grew. Between the years 1929 and 1997, other independent monasteries joined the México City community: Cuba, Argentina, Lyons, Spain and other groups in México. In 1944, the community of Encrucijada, Cuba, was accepted to join the Generalate of México City. Today the Sisters of Mexico City minister in missions in México, Guatemala, El Salvador, Uruguay, Argentina, Peru, Spain, France, Tanzania and Kenya.