This foundation in México was established on December 9, 1903, by two Sisters from Belmont, France and one Sister from Brownsville: Sisters Claire Dubuis, Gonzaga Puillet and Irenee. Because of the persecution and suppression of monasteries in France, the French Sisters decided to join Incarnate Word convents founded in America. At first they stayed with the Sisters of Charity of the Incarnate Word in San Antonio, Texas. From there they went to the convent in Corpus Christi, Texas. Later, in response to a request to open a foundation in Guadalajara, Jalisco, Mother Stanislaus Dedieu decided to send some of the Sisters that had arrived from Belmont. Sisters Marie Claire Dubuis and Marie de Gonzaga Puillet arrived in Guadalajara December 9, 1903. Two other Sisters, one from Brownsville and one from Tabasco, joined them to help with the school there.

As with all the foundations, the Sisters in Guadalajara had their share of hardships, including language differences, weather, different customs, earthquakes, repeated religious persecution, ill health, and poverty. During the Mexican Revolution they endured other dangers and had to live with their families for a while. Some were even imprisoned for days. Yet, the community grew, and numerous foundations and missions were established. About 1968 the Guadalajara community united with Querétero, Cajititlán and Ocotlán. This congregation was officially recognized in 1970.

In 1948, the Sisters from Lyons, France, asked the community in Guadalajara to help make a foundation in Madrid. Sisters María Angela Ledesma, María de la Paz Jiménez, and María del Carmen de Alba were sent there.

Within several years, the Lyons community invited the community of Madrid to join in a union. On July 26, 1955, a new Congregation was formed with the houses of Lyons, France, Madrid, Spain, Guadalajara and Querétaro, México. In 1968, a new foundation was established in Ocotlán, Jalisco; and in 1969, another was founded in Cajititlán, Jalisco. Finally, in 1970, the houses of Lyons, Madrid, and Puente la Reina joined the Generalate in México City; and the houses of Guadalajara, Cajititlán and Ocotlán joined as one. Since then, the Sisters founded other houses in Tuxtla Gutierrez, Chiapas, Rivera de Guadalupe, Jalisco, and Teocelo, Veracruz.